QPCR System

Model Number

Mx3005P

Instrument

• Excitation: Quartz tungsten-halogen source lamp, 5 position excitation filter wheel
  
• Detector: Single scanning photomultiplier tube (PMT), 5 position detection filter wheel
  
• Filters: Customizable filter wheel with five (5) user-selected filters (excitation and detection)*
  
• Thermal system: Solid state, Peltier-based, 96-well block thermal system
  
• Sample format: Standard 96-well plates, 8-strip tubes, 200µl tubes, optimized for 25µl reactions
  
• Embedded computer collects and saves experiment data during power failure or computer crash

*Choice of filters includes: ALEXA Fluor® 350 (350nm-440nm), FAM™/SYbr® Green I (492nm-516nm), TET (517nm-538nm), HEX™/JOE™/VIC™ (535nm-555nm), Cy3™ (545nm-568nm), TAMRA™ (556nm-580nm), ROX™/Texas Red® (585nm-610nm), and Cy5™ (635nm-665nm).

System Specifications

• Linear dynamic range: 10 orders of magnitude, standard curve method
  
• Excitation range: 350 to 750 nm
  
• Emission range: 350 to 700 nm
  
• Temperature uniformity: +/- 0.25 ˚C at 72 ˚C
  
• Amplification rate: standard 40 cycle 2-step QPCR reaction completed in 90 minutes; the same reaction is completed in 60 minutes using FullVelocity™ SYBR® QPCR & QRT-PCR reagents (without dissociation)
  
• Discrimination: distinguish samples with 5,000 and 10,000 template copies with a 99.7% confidence level
  
• Detection down to single-copy equivalent

Dimensions: 13" (33cm) W x 18" (46cm) D x 17"(43 cm) H, Weight: 44 lbs. (20 kg)
Power: 100 to 230 VAC +/- 10%, 50/60 Hz

MxPro™ QPCR Software

• Multiplex up to 5 dyes simultaneously , customer-selected filter set
  
• Independent filter wheel alignment feature enables using FRET chemistry applications
  
• Multiplex plate setup does not require layering (no switching required between dye layers)
  
• Perform multiple sub-experiments on a single plate; data available as raw fluorescence data
  
• Each experiment type includes default setup and automatically displays appropriate analysis view
  
• Ability to start a run in one step using experiment type defaults
  
• Monitor real-time amplification plots and data analysis during a PCR run
• Flexible analysis options: amplification plots, scatter plots, standard curve charts, fluorescence intensity, final call results, melting curve, annealing range and text report
• View all dyes for multiplex experiments on one amplification plot and/or standard curve chart or view individual plots for each individual dye
• Melting curve analysis with automatic melting temperature (Tm) calculation
• Allelic discrimination by threshold cycle or by endpoint fluorescence values with auto-call feature
• Export chart and text report data directly from display screen to Microsoft® Excel, PowerPoint, text and image files
• Software can link up to six (6) Mx3005P and Mx300P instruments with one computer